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Drivers and consequences of soil water preferential flow

Continental scale (40 sites across the United States) & Konza Prairie Biological Station (Northeastern KS)
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Drivers of PF: In a collaboration with a large group of researchers from around the world, we are using high resolution soil moisture data from sites across the United States to assess the probability of PF occurrence based on climate, soil characteristics, and root distributions. In particular, we are interested in understanding how the drivers of PF vary with soil depth and the consequences of PF depth distributions for recharge, water storage, and solute transport. Collaborators for this project include Pam Sullivan (OSU), Matthias Sprenger (Berkeley National Lab), Bonan Li (OSU), Sharon Billings (KU), and many others within the AI4PF working group.

Land-cover change: In work led by Karla Jarecke (CO School of Mines), we are assessing how woody encroachment in a mesic grassland alters the frequency of PF occurrence, the depth of infiltration of water, and long-term drying dynamics using a combination of high resolution soil moisture measurements, electrical resistivity imaging (ERI), and long-term soil moisture records. Pictured (): an irrigation experiment at Konza Prairie Biological station led by Karla Jarecke in which repeated ERI measurements were taken during and after irrigation in adjacent grass-covered and shrub-covered plots to assess the impacts of shrub encroachment on infiltration dynamics. Additional collaborators on this project include Pam Sullivan (OSU), Kamini Singha (CO School of Mines), Matt Kirk (KSU), Daniel Hirmas (TTU), Sharon Billings (KU), Lejo Flores (Boise State), Li Li (Penn State), Hoori Ajami (UC Riverside), and Mark Dumont (CO School of Mines).

Related Publications:

Jarecke, K.M., Zhang, X, Keen, R.M., Dumont, M., Li, B., Sadayappan, K., Moreno, V., Ajami, H., Billings, S.A., Flores, A.N., Hirmas, D.R., Kirk, M.F., Li, L.,

            Nippert, J.B., Singha, K., Sullivan, P.L., 2024. Woody encroachment modifies subsurface structure and hydrological function. Ecohydrology, 

            p.e2731. https://doi.org/10.1002/eco.2731

Quantifying soil nutrient liberation by root exudates across the United States

Continental scale (8 sites across the United States)
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In this project, we are using synthetic exudate cocktails to assess the efficiency of multiple root exudates (i.e., organic acids vs. enzymes) at extracting soil phosphorous, and how this varies both (1) across sites with different soil types, climates, and vegetation cover, and (2) with depth through soil profiles. Recent work has improved our ability to model exudate-related carbon fluxes on a global scale, but we still have a very coarse understanding of the nutrient 'payback' for investment in these exudates. This project aims to fill that gap by assessing exudate phosphorous-extraction efficiency across eight sites in the United States.

Collaborators for this project include Sharon Billings (KU), Pam Sullivan (OSU), Li Li (Penn State), Hoori Ajami (UC Riverside), and Daniel Hirmas (TTU).

Interactive effects of drought + fire in encroached grassland communities

Konza Prairie Biological Station; Northeastern KS, USA
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The goal of this project is to assess the impacts of combined drivers (drought + fire) on the growth and survival of encroaching woody shrubs in a temperate grassland. Passive rainout shelters (50% rainfall exclusion) were built over intact Cornus drummondii shrub islands in the field in 2017 on neighboring watersheds with different burn frequencies (1-yr vs. 4-yr fire return interval). Leaf-level physiology, growth and biomass production, and depth of water uptake has been tracked for C. drummondii and Andropogon gerardii (dominant C4 grass) since 2019. Pictured () is ShRaMPs (the Shrub Rainout Manipulation Plots) at Konza Prairie Biological Station.

PIs and collaborators for this project include Jesse Nippert (KSU), Kevin Wilcox (UNGC), Kate McCulloh (University of Wisconsin-Madison), Lydia Zeglin (KSU), Kimberly O'Keefe (Northern Michigan University), and Greg Tooley (CSU).

Related Publications:

Keen, R.M., Helliker, B.R., McCulloh, K.A. and Nippert, J.B., 2024. Save or spend? Diverging water‐use strategies of grasses and encroaching clonal

         shrubs. Journal of Ecology, 112(4), pp.870-885. https://doi.org/10.1111/1365-2745.14276

Keen, R.M., Bachle, S., Bartmess, M. and Nippert, J.B., 2024. Combined effects of fire and drought are not sufficient to slow shrub encroachment in

         tallgrass prairie. Oecologia, 204(4), pp.727-742. https://doi.org/10.1007/s00442-024-05526-x

O’Keefe, K., Nippert, J.B., Keen, R.M. and McCulloh, K.A., 2024. Contrasting shrub and grass hydraulic responses to experimental drought. Oecologia,     

         pp.1-11. https://doi.org/10.1007/s00442-024-05543-w

Tooley, E.G., Nippert, J.B., Bachle, S. and Keen, R.M., 2022. Intra-canopy leaf trait variation facilitates high leaf area index and compensatory growth in a

         clonal woody encroaching shrub. Tree Physiology, 42(11), pp.2186-2202. https://doi.org/10.1093/treephys/tpac078

O'Keefe, K., Bachle, S., Keen, R.M., Tooley, E.G. and Nippert, J.B., 2022. Root traits reveal safety and efficiency differences in grasses and shrubs

         exposed to different fire regimes. Functional Ecology, 36(2), pp.368-379. https://doi.org/10.1111/1365-2435.13972

Impacts of changing climate and land-cover on grassland ecohydrology

Konza Prairie Biological Station; Northeastern KS, USA
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This research focuses on the impacts of land-cover change (specifically woody encroachment in temperate grasslands) on key water fluxes over time. Vegetation forms a pipeline for water movement from the atmosphere to the subsurface, and vice-versa. Large-scale changes in vegetation composition, particularly when a shift in dominant functional type (i.e., grasses to woody shrubs) occurs, can have substantial impacts on how water cycles through the system. Differences in water-use strategies, root system architecture, and functional rooting depth between functional types result in unique impacts on evapotranspiration, infiltration of water through the soil, and canopy/litter interception of precipitation. These projects largely focus on the contribution of woody encroachment to observed declines in water yield in a mesic grassland in northeastern Kansas. 

PIs and collaborators for these projects include Pam Sullivan (OSU), Jesse Nippert (KSU), Li Li (Penn State), Matt Kirk (KSU), Walter Dodds (KSU), Kayal Saddayappan (Penn State), and Karla Jarecke (CO School of Mines).

Related Publications:

Keen, R.M., Sadayappan, K., Jarecke, K.M., Li, L., Kirk, M.F., Sullivan, P.L. and Nippert, J.B., 2024. Unexpected hydrologic response to ecosystem state

          change in tallgrass prairie. Journal of Hydrology, 643, p.131937. https://doi.org/10.1016/j.jhydrol.2024.131937

Sadayappan, K., Keen, R.M., Jarecke, K.M., Moreno, V., Nippert, J.B., Kirk, M.F., Sullivan, P.L. and Li, L., 2023. Drier streams despite a wetter climate in

          woody-encroached grasslands. Journal of Hydrology, 627, p.130388. https://doi.org/10.1016/j.jhydrol.2023.130388

Dodds, W.K., Ratajczak, R., Keen, R.M., Nippert, J.B., Grudzinski, B., Veach, A., Taylor, J., and Kuhl, A., 2023. Trajectories and state changes of a grassland

          stream and riparian zone after a decade of woody  vegetation removal. Ecological Applications, e2830. https://doi.org/10.1002/eap.2830

Keen, R.M., Nippert, J.B., Sullivan, P.L., Ratajczak, Z., Ritchey, B., O’Keefe, K. and Dodds, W.K., 2022. Impacts of Riparian and Non-riparian Woody

          Encroachment on Tallgrass Prairie Ecohydrology. Ecosystems, pp.1-12. https://doi.org/10.1007/s10021-022-00756-7

Belowground impacts of grassland-to-plantation conversion 

Mariepskop (Great Escarpment); South Africa
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In this project, we assess the belowground impacts of roughly a century of pine (Pinus patula) and eucalypt (Eucalyptus grandis) plantation cover at Mariepskop (1,947 m; pictured) -- one of the highest peaks in the Northern Drakensbergs on the Great Escarpment in South Africa. Historically, this region was dominated by montane grassland and native forest or savanna cover, but grassland areas have almost entirely been converted to plantations or lost to native forest expansion over the last century. We are assessing changes in soil texture, chemistry, and stable carbon isotopes by soil depth to determine how pine and eucalypt cover has altered belowground dynamics through time.

Collaborators for this project include Tony Swemmer (SAEON, Ndlovu Node), and Jesse Nippert (Kansas State University).

Woody root systems in the prairie: Impacts of woody encroachment on carbon storage and biogeochemical cycling

Konza Prairie Biological Station; Northeastern KS, USA
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Woody encroachment results in drastic and obvious changes aboveground, but the changes belowground are often overlooked. The root systems of woody shrubs and trees are typically larger, deeper, and coarser than herbaceous rooting systems. Grasses, in particular, have root systems primarily composed of fine roots in shallow surface soil layers (top 20-30 cm), where they extract the vast majority of their water and nutrients. As woody shrubs and trees spread into historically grassy areas, the shift in depth, diameter, and density of roots impacts carbon storage, biogeochemical cycling, and water fluxes through the soil. We are interested in understanding how the proliferation of woody root systems in grasslands impacts ecosystem-scale cycling of carbon, water, and nutrients. Pictured (): Greg Tooley running the GeoProbe Hydraulic Push-Corer to collect deep soil cores at Konza Prairie.

PIs and collaborators for this project include Jesse Nippert (KSU), Pam Sullivan (OSU), Lydia Zeglin (KSU), and Kimberly O'Keefe (Northern Michigan U.), Greg Tooley (pictured; CSU), and Seton Bachle (LiCOR)

Related Publications:

O'Keefe, K., Bachle, S., Keen, R.M., Tooley, E.G. and Nippert, J.B., 2022. Root traits reveal

          safety and efficiency differences in grasses and shrubs exposed to different fire

          regimes. Functional Ecology, 36(2), pp.368-379.

          https://doi.org/10.1111/1365-2435.13972

Early warning signals for drought-related mortality in the Sierra Nevada Mountains

Soaproot Saddle SSCZO; Central CA, USA
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This project took place in the southern Sierra Nevada Mountains in central California, and centers around the 2012-2015 drought event. This severe, multi-year drought was punctuated by an epidemic-scale outbreak of western pine beetle (Dendroctonus brevicomis), and resulted in widespread mortality of ponderosa pines (Pinus ponderosa), among other dominant canopy tree species. In these studies, we assessed severity of drought stress in surviving trees vs. trees killed by bark beetles during the drought, and found that mortality during this drought event was preceded by decades of increasing sensitivity of tree growth and carbon isotope discrimination (∆13C) to Palmer Drought Severity Index (PDSI) and other hydroclimate variables. We propose that these shifts in sensitivity can serve as early warning signals for future drought-related mortality events in forests of the western United States.

PIs and collaborators for these projects include Steve Voelker (MTU), Barbara Bentz (USDA), and Simon Wang (USU).

Related Publications:

Keen, R.M., Voelker, S.L., Wang, S.Y.S., Bentz, B.J., Goulden, M.L., Dangerfield, C.R., Reed,

        C.C., Hood, S.M., Csank, A.Z., Dawson, T.E. and Merschel, A.G., 2022. Changes in

        tree drought sensitivity provided early warning signals to the California drought and

        forest mortality event. Global Change Biology, 28(3), pp.1119-1132.         

        https://doi.org/10.1111/gcb.15973

Keen, R.M., Voelker, S.L., Bentz, B.J., Wang, S.Y.S. and Ferrell, R., 2020. Stronger influence

        of growth rate than severity of drought stress on mortality of large ponderosa pines

        during the 2012–2015 California drought. Oecologia, 194(3), pp.359-370.

        https://doi.org/10.1007/s00442-020-04771-0

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